Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008]
Catalog No
D12279
Reactivity
Human, Mouse, Rat
Applications
WB
Modification
Acetyl Specific
Source
Polyclonal Rabbit
Dilution
WB: 1:500-1000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Concentration
Storage and Stability
-20°C/1 year
Other Name
H2AFZ; H2AZ; Histone H2A.Z; H2A/z; YM3299
Molecular Weight (Da)
51633
Gene Name
H2AFZ
Protein Name
Histone H2A.Z
Human Gene ID
3015
Human Swiss Prot No.
P0C0S5
Immunogen
Synthetic Peptide of Histone H2A.Z (Acetyl Lys7)
Specificity
The antibody detects endogenous Histone H2A.Z (Acetyl Lys7) protein.
Formulation
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Western blot analysis of extracts from Hela cells, untreated (-) or treated, 1:5000. Secondary antibody(catalogNo.:SA0002) was diluted at 1:20000 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA).
