BCL2 associated agonist of cell death encoded by BAD is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. BCL2 associated agonist of cell death positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of BCL2 associated agonist of cell death is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of BCL2 associated agonist of cell death. Alternative splicing of BAD results in two transcript variants which encode the same isoform.
Catalog No
L0102
Reactivity
Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Cleaved Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
BAD; BBC6; BCL2L8; Bcl2 antagonist of cell death; BAD; Bcl-2-binding component 6; Bcl-2-like protein 8; Bcl2-L-8; Bcl-XL/Bcl-2-associated death promoter
Molecular Weight (Da)
22080
Gene Name
BAD
Protein Name
Bcl2 antagonist of cell death
Human Gene ID
Human Swiss Prot No.
Immunogen
The antiserum was produced against synthesized peptide derived from mouse BAD. AA range:21-70
Specificity
Cleaved-Bad (D71) Polyclonal Antibody detects endogenous levels of fragment of activated Bad protein resulting from cleavage adjacent to D71.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from 293 cells, treated with Etoposide 25uM 60', using BAD (Cleaved-Asp71) Antibody. The lane on the right is blocked with the synthesized peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using BAD (Cleaved-Asp71) Antibody. The picture on the right is blocked with the synthesized peptide.
