This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]
Catalog No
L0158
Reactivity
Human, Rat, Mouse
Applications
WB, ELISA
Modification
Cleaved Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CASP5; ICH3; Caspase-5; CASP-5; ICE(rel)-III; Protease ICH-3; Protease TY
Molecular Weight (Da)
47814
Gene Name
CASP5
Protein Name
Caspase5
Human Gene ID
838
Human Swiss Prot No.
P51878
Immunogen
The antiserum was produced against synthesized peptide derived from human Caspase 5. AA range:102-151
Specificity
Cleaved-Caspase-5 p20 (D121) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-5 p20 protein resulting from cleavage adjacent to D121.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HeLa and A549 cells, treated with etoposide 25uM 24h, using Caspase 5 (p20,Cleaved-Asp121) Antibody. The lane on the right is blocked with the synthesized peptide.
