The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2012]
Catalog No
L0184
Reactivity
Human, Rat, Mouse
Applications
WB, ELISA
Modification
Cleaved Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CTSL1; CTSL; Cathepsin L1; Major excreted protein; MEP
Molecular Weight (Da)
37564
Gene Name
CTSL1
Protein Name
Cathepsin L1
Human Gene ID
1514
Human Swiss Prot No.
P07711
Immunogen
The antiserum was produced against synthesized peptide derived from human CATL1. AA range:239-288
Specificity
Cleaved-Cathepsin L1 HC (T288) Polyclonal Antibody detects endogenous levels of fragment of activated Cathepsin L1 HC protein resulting from cleavage adjacent to T288.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HeLa cells, treated with etoposide 25uM 1h, using CATL1 (heavy chain,Cleaved-Thr288) Antibody. The lane on the right is blocked with the synthesized peptide.
