This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 1
Catalog No
L0192
Reactivity
Human, Rat, Mouse
Applications
WB, ELISA
Modification
Cleaved Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CD97; CD97 antigen; Leukocyte antigen CD97; CD antigen CD97
Molecular Weight (Da)
91869
Gene Name
CD97
Protein Name
CD97 antigen
Human Gene ID
976
Human Swiss Prot No.
P48960
Immunogen
The antiserum was produced against synthesized peptide derived from human CD97 beta. AA range:512-561
Specificity
Cleaved-CD97β (S531) Polyclonal Antibody detects endogenous levels of fragment of activated CD97β protein resulting from cleavage adjacent to S531.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from Jurkat cells, treated with etoposide 25uM 24h, using CD97 beta (Cleaved-Ser531) Antibody. The lane on the right is blocked with the synthesized peptide.
