This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activat
Catalog No
L0203
Reactivity
Human, Rat, Mouse
Applications
WB, ELISA
Modification
Cleaved Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
F12; Coagulation factor XII; Hageman factor; HAF
Molecular Weight (Da)
67818
Gene Name
F12
Protein Name
Coagulation factor XII
Human Gene ID
2161
Human Swiss Prot No.
P00748
Immunogen
The antiserum was produced against synthesized peptide derived from human FA12. AA range:323-372
Specificity
Cleaved-Factor XII HC (R372) Polyclonal Antibody detects endogenous levels of fragment of activated Factor XII HC protein resulting from cleavage adjacent to R372.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from 293 cells, treated with etoposide 25uM 1h, using FA12 (heavy chain,Cleaved-Arg372) Antibody. The lane on the right is blocked with the synthesized peptide.
