This gene encodes a member of the peptidase S10 family of serine carboxypeptidases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate two chains that comprise the heterodimeric active enzyme. This enzyme possesses deamidase, esterase and carboxypeptidase activities and acts as a scaffold in the lysosomal multienzyme complex. Mutations in this gene are associated with galactosialidosis. [provided by RefSeq, Nov 2015]
Catalog No
L0313
Reactivity
Human, Mouse
Applications
WB, ELISA
Modification
Cleaved Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CTSA; PPGB; Lysosomal protective protein; Carboxypeptidase C; Carboxypeptidase L; Cathepsin A; Protective protein cathepsin A; PPCA; Protective protein for beta-galactosidase
Molecular Weight (Da)
54466
Gene Name
CTSA
Protein Name
Lysosomal protective protein
Human Gene ID
5476
Human Swiss Prot No.
P10619
Immunogen
The antiserum was produced against synthesized peptide derived from human PPGB. AA range:277-326
Specificity
Cleaved-Cathepsin A 32k (R326) Polyclonal Antibody detects endogenous levels of fragment of activated Cathepsin A 32k protein resulting from cleavage adjacent to R326.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from 293 and Jurkat cells, treated with etoposide 25uM 1h, using PPGB (32k,Cleaved-Arg326) Antibody. The lane on the right is blocked with the synthesized peptide.
