Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of Extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. [provided by RefSeq, Jul 2008]
Catalog No
L0334
Reactivity
Human, Monkey
Applications
WB, ELISA
Modification
Cleaved Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
MMP27; Matrix metalloproteinase-27; MMP-27
Molecular Weight (Da)
59024
Gene Name
MMP27
Protein Name
Matrix metalloproteinase-27
Human Gene ID
64066
Human Swiss Prot No.
Q9H306
Immunogen
The antiserum was produced against synthesized peptide derived from human MMP27. AA range:80-129
Specificity
Cleaved-MMP-27 (Y99) Polyclonal Antibody detects endogenous levels of fragment of activated MMP-27 protein resulting from cleavage adjacent to Y99.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from COS7 cells, treated with etoposide 25uM 1h, using MMP27 (Cleaved-Tyr99) Antibody. The lane on the right is blocked with the synthesized peptide.
