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Primary Antibodies
AMPKα1/2 (Phospho-Thr183/172) Antibody
AMPKα1/2 (Phospho-Thr183/172) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: A0003
Reactivity:Human,Mouse,Rat,Monkey,Pig,Marsupenaeus japonicus
Applications:IF,WB,IHC,ELISA
Modification:Phospho Specific
Source:Store at -20°C/1 year
Storage and Stability:

$205
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Kinases and Phosphatases
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AMPKα1/2 (Phospho-Thr183/172) Antibody

Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using AMPK alpha (Phospho-Thr172) Antibody

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Product Details
Product Name
AMPKα1/2 (Phospho-Thr183/172) Antibody
Description
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008],
Catalog No
A0003
Reactivity
Human, Mouse, Rat, Monkey, Pig, Marsupenaeus japonicus
Source
Polyclonal, Rabbit,IgG
Modification
Phospho Specific
Applications
IF, WB, IHC, ELISA
Dilution
IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;
Molecular Weight (Da or kDa)
Gene Name
AAPK1/AAPK2
Protein Name
5'-AMP-activated protein kinase catalytic subunit alpha-1/2
Human Gene ID
5562/5563
Human Swiss Prot No.
Q13131 / P54646
Immunogen
The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189
Specificity
Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (17)
Liver-target nanotechnology facilitates berberine to ameliorate cardio-metabolic diseases. Nature Communications Nat Commun. 2019 Apr;10(1):1-16 IF Mouse,Human 1:100 liver tissue hepG2 cell

Author: Available in link provided

Date of Publication : Available in link provided

TNAP inhibition attenuates cardiac fibrosis induced by myocardial infarction through deactivating TGF-β1/Smads and activating P53 signaling pathways. Cell Death & Disease Cell Death Dis. 2020 Jan;11(1):1-15 WB Rat heart

Author: Available in link provided

Date of Publication : Available in link provided

Autophagy and apoptosis mediated nano-copper-induced testicular damage. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2022 Jan;229:113039 WB Rat Testis

Author: Available in link provided

Date of Publication : Available in link provided

Bawei Chenxiang Wan Ameliorates Cardiac Hypertrophy by Activating AMPK/PPAR-α Signaling Pathway Improving Energy Metabolism. Frontiers in Pharmacology Front Pharmacol. 2021 Jun;0:1314 WB Rat Myocardial tissue

Author: Available in link provided

Date of Publication : Available in link provided

SIRT3 deficiency is resistant to autophagy‐dependent ferroptosis by inhibiting the AMPK/mTOR pathway and promoting GPX4 levels. JOURNAL OF CELLULAR PHYSIOLOGY J Cell Physiol. 2020 Nov;235(11):8839-8851 WB Human 1:2000 HTR8/SVeno cell

Author: Available in link provided

Date of Publication : Available in link provided

p53 Mediates Colistin-Induced Autophagy and Apoptosis in PC-12 Cells. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY Antimicrob Agents Ch. 2016 Sep;60(9):5294-5301 WB Rat 1:1000 PC-12 cell

Author: Available in link provided

Date of Publication : Available in link provided

Effect of metformin on apoptosis, cell cycle arrest migration and invasion of A498 cells. Molecular Medicine Reports 2014 Mar 31 WB Human A498 cell

Author: Available in link provided

Date of Publication : Available in link provided

Outer membrane protein a in Acinetobacter baumannii induces autophagy through mTOR signalling pathways in the lung of SD rats. BIOMEDICINE & PHARMACOTHERAPY Biomed Pharmacother. 2021 Mar;135:111034 IF,IHC,WB Rat 1:100,1: 500,1:100 Lung WTRL1 cell

Author: Available in link provided

Date of Publication : Available in link provided

Autophagy was activated against the damages of placentas caused by nano-copper oral exposure. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY Ecotox Environ Safe. 2021 Sep;220:112364 WB Rat 1:1000 Placentas

Author: Available in link provided

Date of Publication : Available in link provided

Metformin ameliorates bladder dysfunction in a rat model of partial bladder outlet obstruction. AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY Am J Physiol-Renal. 2021 Mar;(Articles in Press): WB，IF，IHC Rat 1 : 400，1:1000 Bladder tissues BSMCs

Author: Available in link provided

Date of Publication : Available in link provided

Overexpression of ATG4a promotes autophagy and proliferation, and inhibits apoptosis in lens epithelial cells via the AMPK and Akt pathways. Molecular Medicine Reports Mol Med Rep. 2020 Aug;22(2):1295-1302 WB Human 1 : 1000 Lens epithelial cells

Author: Available in link provided

Date of Publication : Available in link provided

Bawei Chenxiang Wan Ameliorates Cardiac Hypertrophy by Activating AMPK/PPAR-α Signaling Pathway Improving Energy Metabolism. Frontiers in Pharmacology Front Pharmacol. 2021 Jun;0:1314 WB Rat Myocardial tissue

Author: Available in link provided

Date of Publication : Available in link provided

Enhancement of BCAT2-Mediated Valine Catabolism Stimulates β-Casein Synthesis via the AMPK-mTOR Signaling Axis in Bovine Mammary Epithelial Cells JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Zhaoyu Han WB Bovine

Author: Available in link provided

Date of Publication : Available in link provided

The role of AMPK-Sirt1-autophagy pathway in the intestinal protection process by propofol against regional ischemia/reperfusion injury in rats INTERNATIONAL IMMUNOPHARMACOLOGY Mao-Hua Wang IF Rat

Author: Available in link provided

Date of Publication : Available in link provided

WSSV exploits AMPK to activate mTORC2 signaling for proliferation by enhancing aerobic glycolysis Communications Biology Jin-Xing Wang WB,IHC Marsupenaeus japonicus heart,hepatopancreas,gills,stomach,intestines hemocytes

Author: Available in link provided

Date of Publication : Available in link provided

New fungal protein from Pleurotus ferulae lanzi induces AMPK-mediated autophagy and G1-phase cell cycle arrest in A549 lung cancer cells. Zhao-Kun Liu WB,IHC Mouse,Human 1:500,1:150 A549 cell-Xenograft A549 cell

Author: Available in link provided

Date of Publication : Available in link provided

Blockage of EGFR/AKT and mevalonate pathways synergize the antitumor effect of temozolomide by reprogramming energy metabolism in glioblastoma Cancer Communications Xiaoteng Cui WB Human 1:500 TBD0220 cell,U-87 MG cell

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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