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> Products > Primary Antibodies > AMPKα1/2 (Phospho-Thr183/172) Antibody

AMPKα1/2 (Phospho-Thr183/172) Antibody

Overview Description
Datasheet SDS
   Kinases and Phosphatases   
Catalog No : A0003
  • Reactivity
    Human,Mouse,Rat,Monkey,Pig
  • Applications
    IF/ICC,WB,IHC-p,ELISA
  • Modification
    Phospho Specific
  • Source
    Polyclonal Rabbit
  • Storage and Stability
    -20°C/1 year
  • Size
    50 μl
  • Price/Quantity
    $205
  • Quantity
Add to Cart
Details
  • Product Name
    AMPKα1/2 (Phospho-Thr183/172) Antibody
  • Description
    The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
  • Catalog No
    A0003
  • Reactivity
    Human, Mouse, Rat, Monkey, Pig
  • Applications
    IF/ICC, WB, IHC-p, ELISA
  • Modification
    Phospho Specific
  • Source
    Polyclonal Rabbit
  • Dilution
    IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
  • Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • Concentration
    1 mg/ml
  • Storage and Stability
    -20°C/1 year
  • Other Name
    PRKAA1; AMPK1; 5'-AMP-activated protein kinase catalytic subunit alpha-1; AMPK subunit alpha-1; Acetyl-CoA carboxylase kinase; ACACA kinase; Hydroxymethylglutaryl-CoA reductase kinase; HMGCR kinase; Tau-protein kinase PRKAA1; PRKAA2; AMPK;
  • Molecular Weight (Da)
    62808/62320
  • Gene Name
    AAPK1/AAPK2
  • Protein Name
    5'-AMP-activated protein kinase catalytic subunit alpha-1/2
  • Human Gene ID
    5562/5563
  • Human Swiss Prot No.
    Q13131/P54646
  • Immunogen
    The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189
  • Specificity
    Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody detects endogenous levels of AMPKα1/2 protein only when phosphorylated at T183/172.
  • Formulation
    Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Application Data
Western blot analysis of lysates from COS7 cells, using AMPK alpha (Phospho-Thr172) Antibody. The lane on the right is blocked with the phospho peptide.
Application Data
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using AMPK alpha (Phospho-Thr172) Antibody. The picture on the right is blocked with the phospho peptide.
Application Data
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using AMPK alpha (Phospho-Thr172) Antibody
Application Data
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Application Data
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Application Data
The picture was kindly provided by our customer
Application Data
The picture was kindly provided by our customer
Application Data
Western Blot analysis of KB cells using Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody diluted at 1:500
Application Data
Western Blot analysis of various cells using Phospho-AMPKα1/2 (T183/172) Polyclonal Antibody diluted at 1:500
Application Data
Western Blot analysis of mouse-lung cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour). Cell lysate was extracted by Minute™ Plasma Membrane Protein Isolation and Cell Fractionation Kit(SM-005, Inventbiotech,MN,USA).
Application Data
Immunofluorescence analysis of rat-heart tissue. 1,AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Application Data
Min, Qing-Qing, et al. "Effects of Metformin Combined with Lactoferrin on Lipid Accumulation and Metabolism in Mice Fed with High-Fat Diet." Nutrients 10.11 (2018): 1628.
Application Data
Zhang, Ling, et al. "P53 mediates colistin-induced autophagy and apoptosis in PC-12 cells." Antimicrobial agents and chemotherapy (2016): AAC-00641.
Application Data
Guo, Hui-Hui, et al. "Liver-target nanotechnology facilitates berberine to ameliorate cardio-metabolic diseases." Nature communications 10.1 (2019): 1981.
Application Data
Immunofluorescence analysis of Hela cell. 1,AMPKα1/2 (phospho Thr183/172) Polyclonal Antibody(green) was diluted at 1:200(4° overnight). (red) was diluted at 1:200(4° overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog:SA0665 was diluted at 1:1000(room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 Catalog:SA0834 was diluted at 1:1000(room temperature, 50min).
Application Data
Gao, L., Wang, Ly., Liu, Zq. et al. TNAP inhibition attenuates cardiac fibrosis induced by myocardial infarction through deactivating TGF-β1/Smads and activating P53 signaling pathways. Cell Death Dis 11, 44 (2020)
Application Data
Kang, Min, et al. "Autophagy was activated against the damages of placentas caused by nano-copper oral exposure." Ecotoxicology and Environmental Safety 220 (2021): 112364.
Publications

Primary Antibodies

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    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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