This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013]
Catalog No
A0056
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CEBPA; CCAAT/enhancer-binding protein alpha; C/EBP alpha
Molecular Weight (Da)
37575
Gene Name
CEBPA
Protein Name
CCAAT/enhancer-binding protein alpha
Human Gene ID
1050
Human Swiss Prot No.
P49715
Immunogen
The antiserum was produced against synthesized peptide derived from human C/EBP-alpha around the phosphorylation site of Ser21. AA range:6-55
Specificity
Phospho-C/EBP α (S21) Polyclonal Antibody detects endogenous levels of C/EBP α protein only when phosphorylated at S21.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western Blot analysis of mouse liver, rat liver, hela cells, hela cell treated or untreated by insulin. Primary Antibody was diluted at 1:1000. Secondary antibody(catalogNo.:SA0439 was diluted at 1:10000
Western blot analysis of lysates from HepG2 cells treated with EGF 200ng/ml 5', using C/EBP-alpha (Phospho-Ser21) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using C/EBP-alpha (Phospho-Ser21) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human lung cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
Western blot analysis of lysates from Mouse-liver, Rat-liver, Hela cells treated with 0.01U/ml 30min, using C/EBP α (phospho Ser21) Polyclonal Antibody. Primary Antibody was diluted at 1:1000 4° over night,secondary antibody(ABT cat:SA0436)was diluted at 1:10000, 37° 1hour.