The protein encoded by this gene has a long and a short form, generated by use of alternative translational start codons. The long form is expressed in steroidogenic tissues such as testis, where it converts cholesteryl esters to free cholesterol for steroid hormone production. The short form is expressed in adipose tissue, among others, where it hydrolyzes stored triglycerides to free fatty acids. [provided by RefSeq, Jul 2008]
Catalog No
A0074
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
LIPE; Hormone-sensitive lipase; HSL
Molecular Weight (Da)
116568
Gene Name
LIPE
Protein Name
Hormone-sensitive lipase
Human Gene ID
3991
Human Swiss Prot No.
Q05469
Immunogen
The antiserum was produced against synthesized peptide derived from human HSL around the phosphorylation site of Ser855/554. AA range:520-569
Specificity
Phospho-HSL (S855) Polyclonal Antibody detects endogenous levels of HSL protein only when phosphorylated at S855.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HeLa cells treated with Adriamycin 0.5ng/ml 24h, using HSL (Phospho-Ser855/554) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using HSL (Phospho-Ser855/554) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HSL (Phospho-Ser855/554) Antibody