BCAR1, or CAS, is an Src (MIM 190090) family kinase substrate involved in various cellular events, including migration, survival, transformation, and invasion (Sawada et al., 2006 [PubMed 17129785]).[supplied by OMIM, May 2009]
Catalog No
A0077
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
BCAR1; CAS; CASS1; CRKAS; Breast cancer anti-estrogen resistance protein 1; CRK-associated substrate; Cas scaffolding protein family member 1; p130cas
Molecular Weight (Da)
93362
Gene Name
BCAR1
Protein Name
Breast cancer anti-estrogen resistance protein 1
Human Gene ID
9564
Human Swiss Prot No.
P56945
Immunogen
The antiserum was produced against synthesized peptide derived from human p130 Cas around the phosphorylation site of Tyr410. AA range:376-425
Specificity
Phospho-p130 Cas (Y410) Polyclonal Antibody detects endogenous levels of p130 Cas protein only when phosphorylated at Y410.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from NIH/3T3 cells, using p130 Cas (Phospho-Tyr410) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using p130 Cas (Phospho-Tyr410) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
