The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
Catalog No
A0089
Reactivity
Human, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
STAT2; Signal transducer and activator of transcription 2; p113
Molecular Weight (Da)
97916
Gene Name
STAT2
Protein Name
Signal transducer and activator of transcription 2
Human Gene ID
6773
Human Swiss Prot No.
P52630
Immunogen
The antiserum was produced against synthesized peptide derived from human STAT2 around the phosphorylation site of Tyr690. AA range:656-705
Specificity
Phospho-Stat2 (Y690) Polyclonal Antibody detects endogenous levels of Stat2 protein only when phosphorylated at Y690.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HeLa cells treated with IFN 2500U/ml 30', using STAT2 (Phospho-Tyr690) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using STAT2 (Phospho-Tyr690) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using STAT2 (Phospho-Tyr690) Antibody
Western blot analysis of Stat2 (phospho Tyr690) Polyclonal Antibody, using Hela,Jurkat cell treated or untreated with IFN 2500U/ml 30', 4° over night, secondary antibody(cat: SA0002 was diluted at 1:10000, 37° 1hour.
Western blot analysis of lysates from Hela cells treated with IFN 2500U/ml 30', using Stat2 p-Thr690 Antibody. Primary Antibody was diluted at 1:1000 4° over night,secondary antibody(ABT cat:SA0002)was diluted at 1:10000, 37° 1hour.
