eukaryotic translation initiation factor 4E binding protein 1(EIF4EBP1) Homo sapiens This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]
Catalog No
A0401
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Molecular Weight (Da)
12449
Gene Name
EIF4EBP1
Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
Human Gene ID
1978
Human Swiss Prot No.
Q13541
Immunogen
The antiserum was produced against synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Ser64. AA range:30-79
Specificity
Phospho-4E-BP1 (S64) Polyclonal Antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at S64.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from Jurkat cells treated with Insulin 0.01U/ml 15', using 4E-BP1 (Phospho-Ser64) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using 4E-BP1 (Phospho-Ser64) Antibody. The picture on the right is blocked with the phospho peptide.
Wang, Tao, et al. "RAPTOR promotes colorectal cancer proliferation by inducing mTORC1 and upregulating ribosome assembly factor URB1." Cancer medicine 9.4 (2020): 1529-1543.