The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activtiy is required for cell cycle G1/S transition. This protein has been shown to interact with and be involved in the phosphorylation of tumor suppressor protein Rb. The CDK4 activity associated with this cyclin was reported to be necessary for cell cycle progression through G2 phase into mitosis after UV radiation. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
Catalog No
A0418
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CCND3; G1/S-specific cyclin-D3
Molecular Weight (Da)
32520
Gene Name
CCND3
Protein Name
G1/S-specific cyclin-D3
Human Gene ID
896
Human Swiss Prot No.
P30281
Immunogen
The antiserum was produced against synthesized peptide derived from human Cyclin D3 around the phosphorylation site of Thr283. AA range:243-292
Specificity
Phospho-Cyclin D3 (T283) Polyclonal Antibody detects endogenous levels of Cyclin D3 protein only when phosphorylated at T283.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from K562 cells treated with UV 5', using Cyclin D3 (Phospho-Thr283) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using Cyclin D3 (Phospho-Thr283) Antibody. The picture on the right is blocked with the phospho peptide.
