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Primary Antibodies
E2F-1 (Phospho-Thr433) Antibody
E2F-1 (Phospho-Thr433) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: A0423
Reactivity:Human,Mouse
Applications:WB,ELISA
Modification:Phospho Specific
Source:Store at -20°C/1 year
Storage and Stability:

$205
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E2F-1 (Phospho-Thr433) Antibody

Western blot analysis of lysates from HeLa cells treated with Etoposide 25uM 24h, using E2F1 (Phospho-Thr433) Antibody. The lane on the right is blocked with the phospho peptide.

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Product Details
Product Name
E2F-1 (Phospho-Thr433) Antibody
Description
The protein encoded byThis gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can media
Catalog No
A0423
Reactivity
Human, Mouse
Source
Polyclonal, Rabbit,IgG
Modification
Phospho Specific
Applications
WB, ELISA
Dilution
WB 1:500 - 1:2000. ELISA: 1:5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
E2F1; RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1
Molecular Weight (Da or kDa)
Gene Name
E2F1
Protein Name
Transcription factor E2F1
Human Gene ID
1869
Human Swiss Prot No.
Q01094
Immunogen
The antiserum was produced against synthesized peptide derived from human E2F1 around the phosphorylation site of Thr433. AA range:388-437
Specificity
Phospho-E2F-1 (T433) Polyclonal Antibody detects endogenous levels of E2F-1 protein only when phosphorylated at T433.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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