Fanconi anemia complementation group D2(FANCD2) Homo sapiens The Fanconi anemia complementation group (FANC) currently includes FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The previously defined group FANCH is the same as FANCA. Fanconi anemia is a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. The members of the Fanconi anemia complementation group do not share sequence similarity; they are related by their assembly into a common nuclear Protein complex. This gene encodes the protein for complementation group D2. This protein is monoubiquinated in response to DNA damage, resulting in its localization to nuclear foci with other proteins (BRCA1 AND BRCA2) involved in homology-directed DNA repai
Catalog No
A0427
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
FANCD2; FACD; Fanconi anemia group D2 protein; Protein FACD2
Molecular Weight (Da)
166462
Gene Name
FANCD2
Protein Name
Fanconi anemia group D2 protein
Human Gene ID
2177
Human Swiss Prot No.
Q9BXW9
Immunogen
The antiserum was produced against synthesized peptide derived from human FANCD2 around the phosphorylation site of Ser222. AA range:188-237
Specificity
Phospho-FANCD2 (S222) Polyclonal Antibody detects endogenous levels of FANCD2 protein only when phosphorylated at S222.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HT29 cells treated with Calyculin A 50ng/ml 30', using FANCD2 (Phospho-Ser222) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma, using FANCD2 (Phospho-Ser222) Antibody. The picture on the right is blocked with the phospho peptide.
