The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]
Catalog No
A0431
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
GYS1; GYS; Glycogen [starch] synthase; muscle
Molecular Weight (Da)
83786
Gene Name
GYS1
Protein Name
Glycogen [starch] synthase muscle
Human Gene ID
2997
Human Swiss Prot No.
P13807
Immunogen
The antiserum was produced against synthesized peptide derived from human Glycogen Synthase around the phosphorylation site of Ser645. AA range:611-660
Specificity
Phospho-Glycogen Synthase 1 (S645) Polyclonal Antibody detects endogenous levels of Glycogen Synthase 1 protein only when phosphorylated at S645.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using Glycogen Synthase (Phospho-Ser645) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Glycogen Synthase (Phospho-Ser645) Antibody. The picture on the right is blocked with the phospho peptide.
