The protein encoded by this gene belongs to the NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with, and trapping it in the Cytoplasm. Phosphorylation of serine residues on these proteins by kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B, which translocates to the nucleus to function as a transcription factor. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jul 2011]
Catalog No
A0438
Reactivity
Human, Rat, Mouse
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
NFKBIB; IKBB; TRIP9; NF-kappa-B inhibitor beta; NF-kappa-BIB; I-kappa-B-beta; IkB-B; IkB-beta; IkappaBbeta; Thyroid receptor-interacting protein 9; TR-interacting protein 9; TRIP-9
Molecular Weight (Da)
37771
Gene Name
NFKBIB
Protein Name
NF-kappa-B inhibitor beta
Human Gene ID
4793
Human Swiss Prot No.
Q15653
Immunogen
The antiserum was produced against synthesized peptide derived from human IkappaB-beta around the phosphorylation site of Thr19. AA range:4-53
Specificity
Phospho-IκB-β (T19) Polyclonal Antibody detects endogenous levels of IκB-β protein only when phosphorylated at T19.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from 293 cells treated with TNF-a 20ng/ml 30', using IkappaB-beta (Phospho-Thr19) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
