The protein encoded by this gene is the common beta chain of the high affinity receptor for IL-3, IL-5 and CSF. Defects in this gene have been reported to be associated with protein alveolar proteinosis (PAP). [provided by RefSeq, Jul 2008]
Catalog No
A0444
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CSF2RB; IL3RB; IL5RB; Cytokine receptor common subunit beta; CDw131; GM-CSF/IL-3/IL-5 receptor common beta subunit; CD antigen CD131
Molecular Weight (Da)
97336
Gene Name
CSF2RB
Protein Name
Cytokine receptor common subunit beta
Human Gene ID
1439
Human Swiss Prot No.
P32927
Immunogen
The antiserum was produced against synthesized peptide derived from human IL-3R beta around the phosphorylation site of Tyr593. AA range:559-608
Specificity
Phospho-IL-3Rβ (Y593) Polyclonal Antibody detects endogenous levels of IL-3Rβ protein only when phosphorylated at Y593.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from RAW264.7 cells treated with G-CSF 25ng/ml 15', using IL-3R beta (Phospho-Tyr593) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using IL-3R beta (Phospho-Tyr593) Antibody. The picture on the right is blocked with the phospho peptide.
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
