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Primary Antibodies
PRAS40 (Phospho-Thr246) Antibody
PRAS40 (Phospho-Thr246) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: A0453
Reactivity:Human,Mouse,Rat
Applications:WB,IHC-p,IF(paraffin section),ELISA
Modification:Phospho Specific
Source:Polyclonal Rabbit
Storage and Stability:-20°C/1 year

$205
# Datasheet #
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Tumor Suppressors/Apoptosis
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PRAS40 (Phospho-Thr246) Antibody

Western blot analysis of lysates from HepG2 cells treated with PDGF 50ng/ml 30', using Akt1 S1 (Phospho-Thr246) Antibody. The lane on the right is blocked with the phospho peptide.

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Product Details
Product Name
PRAS40 (Phospho-Thr246) Antibody
Description
AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM, Mar 2008]
Catalog No
A0453
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
AKT1S1; PRAS40; Proline-rich AKT1 substrate 1; 40 kDa proline-rich AKT substrate
Molecular Weight (Da)
27383
Gene Name
AKT1S1
Protein Name
Proline-rich AKT1 substrate 1
Human Gene ID
84335
Human Swiss Prot No.
Q96B36
Immunogen
The antiserum was produced against synthesized peptide derived from human Akt1 S1 around the phosphorylation site of Thr246. AA range:207-256
Specificity
Phospho-PRAS40 (T246) Polyclonal Antibody detects endogenous levels of PRAS40 protein only when phosphorylated at T246.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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PRAS40 (Phospho-Thr246) Antibody

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Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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