This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Catalog No
A0462
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CALD1; CAD; CDM; Caldesmon; CDM
Molecular Weight (Da)
high molecular weight (predominantly in smooth muscles) (120-150 KD) and a low molecular weight (60-90KD)
Gene Name
CALD1
Protein Name
Caldesmon
Human Gene ID
800
Human Swiss Prot No.
Q05682
Immunogen
The antiserum was produced against synthesized peptide derived from human Caldesmon around the phosphorylation site of Ser789. AA range:744-793
Specificity
Phospho-Caldesmon (S789) Polyclonal Antibody detects endogenous levels of Caldesmon protein only when phosphorylated at S789.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HeLa cells treated with EGF 200ng/ml 30', using Caldesmon (Phospho-Ser789) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Caldesmon (Phospho-Ser789) Antibody. The picture on the right is blocked with the phospho peptide.