Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CD247; CD3Z; T3Z; TCRZ; T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD antigen CD247
Molecular Weight (Da)
18696
Gene Name
CD247
Protein Name
T-cell surface glycoprotein CD3 zeta chain
Human Gene ID
919
Human Swiss Prot No.
P20963
Immunogen
The antiserum was produced against synthesized peptide derived from human CD3 zeta around the phosphorylation site of Tyr142. AA range:111-160
Specificity
Phospho-CD3 ζ (Y142) Polyclonal Antibody detects endogenous levels of CD3 ζ protein only when phosphorylated at Y142.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
