This gene encodes a member of the ETS family of transcription factors, which are defined by the presence of a conserved ETS DNA-binding domain that recognizes the core consensus DNA sequence GGAA/T in target genes. These proteins function either as transcriptional activators or repressors of numerous genes, and are involved in stem cell development, cell senescence and death, and tumorigenesis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
Catalog No
A0478
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
ETS1; EWSR2; Protein C-ets-1; p54
Molecular Weight (Da)
50202
Gene Name
ETS1
Protein Name
Protein C-ets-1
Human Gene ID
2113
Human Swiss Prot No.
P14921
Immunogen
The antiserum was produced against synthesized peptide derived from human ETS1 around the phosphorylation site of Thr38. AA range:11-60
Specificity
Phospho-Ets-1 (T38) Polyclonal Antibody detects endogenous levels of Ets-1 protein only when phosphorylated at T38.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HeLa cells treated with Serum 20% 15', using ETS1 (Phospho-Thr38) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using ETS1 (Phospho-Thr38) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ETS1 (Phospho-Thr38) Antibody
Author:
Wen‐Ke Wang Qing‐Hua Lu Jia‐Ning Zhang Ben Wang Xiang‐Juan Liu Feng‐Shuang An Wei‐Dong Qin Xue‐Ying Chen Wen‐Qian Dong Cheng Zhang Yun Zhang Ming‐Xiang Zhang
