E1A binding protein p300(EP300) Homo sapiens This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]
Catalog No
A0528
Reactivity
Human, Mouse, Rat
Applications
IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
EP300; P300; Histone acetyltransferase p300; p300 HAT; E1A-associated protein p300
Molecular Weight (Da)
264144
Gene Name
EP300
Protein Name
Histone acetyltransferase p300
Human Gene ID
2033
Human Swiss Prot No.
Q09472
Immunogen
The antiserum was produced against synthesized peptide derived from human p300 around the phosphorylation site of Ser89. AA range:55-104
Specificity
Phospho-p300 (S89) Polyclonal Antibody detects endogenous levels of p300 protein only when phosphorylated at S89.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human thyroid gland, using p300 (Phospho-Ser89) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p300 (Phospho-Ser89) Antibody