This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the Cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Catalog No
A0581
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
SYN1; Synapsin-1; Brain protein 4.1; Synapsin I
Molecular Weight (Da)
73988
Gene Name
SYN1
Protein Name
Synapsin-1
Human Gene ID
6853
Human Swiss Prot No.
P17600
Immunogen
The antiserum was produced against synthesized peptide derived from human Synapsin1 around the phosphorylation site of Ser62. AA range:26-75
Specificity
Phospho-Synapsin I (S62) Polyclonal Antibody detects endogenous levels of Synapsin I protein only when phosphorylated at S62.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HeLa cells treated with Anisomycin 25ug/ml 30', using Synapsin1 (Phospho-Ser62) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Synapsin1 (Phospho-Ser62) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Synapsin1 (Phospho-Ser62) Antibody