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Primary Antibodies
B23 (Phospho-Thr199) Antibody
B23 (Phospho-Thr199) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: A0694
Reactivity:Human,Mouse,Rat
Applications:WB,IHC-p,IF/ICC,ELISA
Modification:Phospho Specific
Source:Polyclonal Rabbit
Storage and Stability:-20°C/1 year

$205
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B23 (Phospho-Thr199) Antibody

Western blot analysis of B23 (phospho Thr199) Polyclonal Antibody, using Hela, HepG2 cell treated or untreated with forskolin 40nM 30', 4° over night, secondary antibody(cat: SA0002 was diluted at 1:10000, 37° 1hour.

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Product Details
Product Name
B23 (Phospho-Thr199) Antibody
Description
This gene encodes a phosphoprotein which moves between the nucleus and the Cytoplasm. The gene product is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners have been characterized, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in this gene are associated with acute myeloid leukemia. More than a dozen pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Nov 2009]
Catalog No
A0694
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
NPM1; NPM; Nucleophosmin; NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin
Molecular Weight (Da)
32575
Gene Name
NPM1
Protein Name
Nucleophosmin
Human Gene ID
4869
Human Swiss Prot No.
P06748
Immunogen
The antiserum was produced against synthesized peptide derived from human NPM around the phosphorylation site of Thr199. AA range:171-220
Specificity
Phospho-B23 (T199) Polyclonal Antibody detects endogenous levels of B23 protein only when phosphorylated at T199.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Primary Antibodies

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    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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