Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis. [provided by RefSeq, Jul 2008]
Catalog No
A0793
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
HDAC1; RPD3L1; Histone deacetylase 1; HD1
Molecular Weight (Da)
55103
Gene Name
HDAC1
Protein Name
Histone deacetylase 1
Human Gene ID
3065
Human Swiss Prot No.
Q13547
Immunogen
The antiserum was produced against synthesized peptide derived from human HDAC1 around the phosphorylation site of Ser421. AA range:387-436
Specificity
Phospho-HDAC1 (S421) Polyclonal Antibody detects endogenous levels of HDAC1 protein only when phosphorylated at S421.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using HDAC1 (Phospho-Ser421) Antibody. The lane on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HDAC1 (Phospho-Ser421) Antibody