The protein encoded by this gene is a G protein-coupled receptor that is expressed in the parathyroid hormone (PTH)-producing chief cells of the parathyroid gland, and the cells lining the kidney tubule. It senses small changes in circulating calcium concentration and couples this information to intracellular signaling pathways that modify PTH secretion or renal cation handling, thus this protein plays an essential role in maintaining mineral ion homeostasis. Mutations in this gene cause familial hypocalciuric hypercalcemia, familial, isolated hypoparathyroidism, and neonatal severe primary hyperparathyroidism. [provided by RefSeq, Jul 2008]
Catalog No
A0828
Reactivity
Human, Mouse, Rat
Applications
WB, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
The antiserum was produced against synthesized peptide derived from human Calcium Sensing Receptor around the phosphorylation site of Thr888. AA range:854-903
Specificity
Phospho-CaSR (T888) Polyclonal Antibody detects endogenous levels of CaSR protein only when phosphorylated at T888.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from LOVO cells, using Calcium Sensing Receptor (Phospho-Thr888) Antibody. The lane on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HeLa cells, using Calcium Sensing Receptor (Phospho-Thr888) Antibody. The picture on the right is blocked with the phospho peptide.
