This gene encodes a member of the EF-hand calcium-binding protein family. It is one of three genes which encode an identical calcium binding protein which is one of the four subunits of phosphorylase kinase. Two pseudogenes have been identified on chromosome 7 and X. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]
Catalog No
A0829
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
The antiserum was produced against synthesized peptide derived from human Calmodulin around the phosphorylation site of Thr79 and Ser81. AA range:46-95
Specificity
Phospho-Calmodulin (T80/S82) Polyclonal Antibody detects endogenous levels of Calmodulin protein only when phosphorylated at T80/S82.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from Jurkat cells treated with Insulin 0.01U/ml 15', using Calmodulin (Phospho-Thr79+Ser81) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Calmodulin (Phospho-Thr79+Ser81) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of HepG2 cells, using Calmodulin (Phospho-Thr79+Ser81) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Calmodulin (Phospho-Thr79+Ser81) Antibody
