This gene encodes the beta subunit of casein kinase II, a ubiquitous protein kinase which regulates metabolic pathways, signal transduction, transcription, translation, and replication. The enzyme is composed of three subunits, alpha, alpha prime and beta, which form a tetrameric holoenzyme. The alpha and alpha prime subunits are catalytic, while the beta subunit serves regulatory functions. The enzyme localizes to the endoplasmic reticulum and the Golgi apparatus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2013]
Catalog No
A0869
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CSNK2B; CK2N; G5A; Casein kinase II subunit beta; CK II beta; Phosvitin; Protein G5a
Molecular Weight (Da)
24942
Gene Name
CSNK2B
Protein Name
Casein kinase II subunit beta
Human Gene ID
1460
Human Swiss Prot No.
P67870
Immunogen
The antiserum was produced against synthesized peptide derived from human CKII-beta around the phosphorylation site of Ser209. AA range:166-215
Specificity
Phospho-Casein Kinase IIβ (S209) Polyclonal Antibody detects endogenous levels of Casein Kinase IIβ protein only when phosphorylated at S209.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HUVEC cells, using CKII-beta (Phospho-Ser209) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using CKII-beta (Phospho-Ser209) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CKII-beta (Phospho-Ser209) Antibody