The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Catalog No
A0930
Reactivity
Human, Mouse
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
FOSB; G0S3; Protein fosB; G0/G1 switch regulatory protein 3
Molecular Weight (Da)
35928
Gene Name
FOSB
Protein Name
Protein fosB
Human Gene ID
2354
Human Swiss Prot No.
P53539
Immunogen
The antiserum was produced against synthesized peptide derived from human FosB around the phosphorylation site of Ser27. AA range:12-61
Specificity
Phospho-Fos B (S27) Polyclonal Antibody detects endogenous levels of Fos B protein only when phosphorylated at S27.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunofluorescence analysis of HUVEC cells treated with EGF 200nM 5', using FosB (Phospho-Ser27) Antibody. The picture on the right is blocked with the phospho peptide.
