Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]
Catalog No
A0939
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
HDAC3; Histone deacetylase 3; HD3; RPD3-2; SMAP45
Molecular Weight (Da)
48848
Gene Name
HDAC3
Protein Name
Histone deacetylase 3
Human Gene ID
8841
Human Swiss Prot No.
O15379
Immunogen
The antiserum was produced against synthesized peptide derived from human HDAC3 around the phosphorylation site of Ser424. AA range:379-428
Specificity
Phospho-HDAC3 (S424) Polyclonal Antibody detects endogenous levels of HDAC3 protein only when phosphorylated at S424.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human skin, using HDAC3 (Phospho-Ser424) Antibody. The picture on the right is blocked with the phospho peptide.