This gene (IFNGR1) encodes the ligand-binding chain (alpha) of the gamma interferon receptor. Human interferon-gamma receptor is a heterodimer of IFNGR1 and IFNGR2. A genetic variation in IFNGR1 is associated with susceptibility to Helicobacter pylori infection. In addition, defects in IFNGR1 are a cause of mendelian susceptibility to mycobacterial disease, also known as familial disseminated atypical mycobacterial infection. [provided by RefSeq, Jul 2008]
Catalog No
A0953
Reactivity
Human, Mouse, Rat, Monkey
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
The antiserum was produced against synthesized peptide derived from human Interferon-gamma Receptor alpha around the phosphorylation site of Tyr457. AA range:431-480
Specificity
Phospho-IFN-γRα (Y457) Polyclonal Antibody detects endogenous levels of IFN-γRα protein only when phosphorylated at Y457.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from COS7 cells, using Interferon-gamma Receptor alpha (Phospho-Tyr457) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human brain, using Interferon-gamma Receptor alpha (Phospho-Tyr457) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of A549 cells, using Interferon-gamma Receptor alpha (Phospho-Tyr457) Antibody. The picture on the right is blocked with the phospho peptide.