nuclear factor kappa B subunit 2(NFKB2) Homo sapiens This gene encodes a subunit of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB complex is expressed in numerous cell types and functions as a central activator of genes involved in inflammation and immune function. The protein encoded by this gene can function as both a transcriptional activator or repressor depending on its dimerization partner. The p100 full-length protein is co-translationally processed into a p52 active form. Chromosomal rearrangements and translocations of this locus have been observed in B cell lymphomas, some of which may result in the formation of fusion proteins. There is a pseudogene for this gene on chromosome 18. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]
Catalog No
A1104
Reactivity
Human, Mouse
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Western blot analysis of lysates from RAW264.7 cells treated with EGF 200ng/ml 30', using NF-kappaB p100 (Phospho-Ser872) Antibody. Primary Antibody was diluted at 1:1000 4° over night,secondary antibody(ABT cat:SA0439)was diluted at 1:10000, 37° 1hour.