This gene is a member of the TNF-receptor superfamily. The encoded protein is a receptor on antigen-presenting cells of the immune system and is essential for mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Mutations affecting this gene are the cause of autosomal recessive hyper-IgM immunodeficiency type 3 (HIG
Catalog No
A1129
Reactivity
Human, Mouse
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CD40; TNFRSF5; Tumor necrosis factor receptor superfamily member 5; B-cell surface antigen CD40; Bp50; CD40L receptor; CDw40; CD antigen CD40
Molecular Weight (Da)
30619
Gene Name
CD40
Protein Name
Tumor necrosis factor receptor superfamily member 5
Human Gene ID
958
Human Swiss Prot No.
P25942
Immunogen
The antiserum was produced against synthesized peptide derived from human TNFRSF5 around the phosphorylation site of Thr254. AA range:220-269
Specificity
Phospho-CD40 (T254) Polyclonal Antibody detects endogenous levels of CD40 protein only when phosphorylated at T254.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from COLO205 cells treated with Insulin 0.01U/ml 15', using TNFRSF5 (Phospho-Thr254) Antibody. The lane on the right is blocked with the phospho peptide.
