The protein encoded by this gene is a 47 kDa Cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]
Catalog No
A1161
Reactivity
Human, Mouse, Rat, Cow
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
The antiserum was produced against synthesized peptide derived from human Neutrophil Cytosol Factor 1 around the phosphorylation site of Ser328. AA range:301-350
Specificity
Phospho-p47-phox (S328) Polyclonal Antibody detects endogenous levels of p47-phox protein only when phosphorylated at S328.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human tonsil, using Neutrophil Cytosol Factor 1 (Phospho-Ser328) Antibody. The picture on the right is blocked with the phospho peptide.
Author:
Siyuan Liu, Xiaobing Li, Xiaohan Zhou, Juan J. Loor, Qianming Jiang, Xiancheng Feng, Yuchen Yang, Lin Lei, Xiliang Du, Xinwei Li, Wang Zhe, Yuxiang Song, Guowen Liu