This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
Catalog No
A1168
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
NR4A1; GFRP1; HMR; NAK1; Nuclear receptor subfamily 4 group A member 1; Early response protein NAK1; Nuclear hormone receptor NUR/77; Nur77; Orphan nuclear receptor HMR; Orphan nuclear receptor TR3; ST-59; Testicular receptor 3
Molecular Weight (Da)
64463
Gene Name
NR4A1
Protein Name
Nuclear receptor subfamily 4 group A member 1
Human Gene ID
3164
Human Swiss Prot No.
P22736
Immunogen
The antiserum was produced against synthesized peptide derived from human Nuclear Receptor NR4A1 around the phosphorylation site of Ser351. AA range:317-366
Specificity
Phospho-Nur77 (S351) Polyclonal Antibody detects endogenous levels of Nur77 protein only when phosphorylated at S351.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Nuclear Receptor NR4A1 (Phospho-Ser351) Antibody. The picture on the right is blocked with the phospho peptide.