This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. Expression is induced by phytohemagglutinin in human lymphocytes and by serum stimulation of arrested fibroblasts. The encoded protein acts as a nuclear transcription factor. Translocation of the protein from the nucleus to mitochondria induces apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
Catalog No
A1168sec
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
WB 1:500-2000, ELISA(peptide)1:5000-20000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
Nuclear receptor subfamily 4 group A member 1 (Early response protein NAK1) (Nuclear hormone receptor NUR/77) (Nur77) (Orphan nuclear receptor HMR) (Orphan nuclear receptor TR3) (ST-59) (Testicular receptor 3)
Molecular Weight (Da)
Gene Name
NR4A1 GFRP1 HMR NAK1
Protein Name
Nuclear Receptor NR4A1 (Phospho-Ser351)
Human Gene ID
3164
Human Swiss Prot No.
P22736
Immunogen
Synthesized pospho peptide derived from human Nuclear Receptor NR4A1 (Phospho-Ser351)
Specificity
This antibody detects endogenous pospho levels of human Nuclear Receptor NR4A1 (Phospho-Ser351)
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: SA0439)was diluted at 1:10000, 37° 1hour.
