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DNA Ligase IV (Phospho-Thr650) Antibody
DNA Ligase IV (Phospho-Thr650) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: A1197
Reactivity:Human,Rat,Mouse
Applications:IHC,IF,ELISA
Modification:Phospho Specific
Source:Store at -20°C/1 year
Storage and Stability:

$205
Product Details
Product Name
DNA Ligase IV (Phospho-Thr650) Antibody
Description
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008],
Catalog No
A1197
Reactivity
Human, Rat, Mouse
Source
Polyclonal, Rabbit,IgG
Modification
Phospho Specific
Applications
IHC, IF, ELISA
Dilution
IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
LIG4; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4
Molecular Weight (Da or kDa)
104kD
Gene Name
LIG4
Protein Name
DNA ligase 4
Human Gene ID
3981
Human Swiss Prot No.
P49917
Immunogen
The antiserum was produced against synthesized peptide derived from human DNA Ligase 4 around the phosphorylation site of Thr650. AA range:616-665
Specificity
Phospho-DNA Ligase IV (T650) Polyclonal Antibody detects endogenous levels of DNA Ligase IV protein only when phosphorylated at T650.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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