The protein encoded by this gene is closely related to STAM, an adaptor protein involved in the downstream signaling of cytokine receptors, both of which contain a SH3 domain and the immunoreceptor tyrosine-based activation motif (ITAM). Similar to STAM, this protein acts downstream of JAK kinases, and is phosphorylated in response to cytokine stimulation. This protein and STAM thus are thought to exhibit compensatory effects on the signaling pathway downstream of JAK kinases upon cytokine stimulation. [provided by RefSeq, Jul 2008]
Catalog No
A1237
Reactivity
Human, Mouse
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
STAM2; HBP; Signal transducing adapter molecule 2; STAM-2; Hrs-binding protein
Molecular Weight (Da)
58164
Gene Name
STAM2
Protein Name
Signal transducing adapter molecule 2
Human Gene ID
8986
Human Swiss Prot No.
O75886
Immunogen
The antiserum was produced against synthesized peptide derived from human STAM2 around the phosphorylation site of Tyr192. AA range:161-210
Specificity
Phospho-STAM2 (Y192) Polyclonal Antibody detects endogenous levels of STAM2 protein only when phosphorylated at Y192.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from NIH/3T3 cells treated with EGF 200ng/ml 30', using STAM2 (Phospho-Tyr192) Antibody. The lane on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using STAM2 (Phospho-Tyr192) Antibody
