eukaryotic translation initiation factor 4E binding protein 1(EIF4EBP1) Homo sapiens This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008]
Catalog No
A7001
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
EIF4EBP1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E-BP1; eIF4E-binding protein 1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; PHAS-I
Molecular Weight (Da)
12449
Gene Name
EIF4EBP1
Protein Name
Eukaryotic translation initiation factor 4E-binding protein 1
Human Gene ID
1978
Human Swiss Prot No.
Q13541
Immunogen
The antiserum was produced against synthesized peptide derived from human 4E-BP1 around the phosphorylation site of Thr36. AA range:4-53
Specificity
Phospho-4E-BP1 (T37) Polyclonal Antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at T37.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from MDA-MB-435 cells treated with EGF 200ng/ml 30', using 4E-BP1 (Phospho-Thr36) Antibody. The lane on the left is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using 4E-BP1 (Phospho-Thr36) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using 4E-BP1 (Phospho-Thr36) Antibody
Gao, Hai-na, et al. "Leucine and histidine independently regulate milk protein synthesis in bovine mammary epithelial cells via mTOR signaling pathway 亮氨酸和组氨酸通过 mTOR 信号通路调控奶牛腺上皮细胞中酪蛋白的合成." Journal of Zhejiang University-SCIENCE B 16.6 (2015): 560-572.
