The product of this gene belongs to the serine/threonine protein kinases family, and to the Ca(2+)/calmodulin-dependent protein kinases subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. This calcium calmodulin-dependent protein kinase is composed of four different chains: alpha, beta, gamma, and delta. The alpha chain encoded by this gene is required for hippocampal long-term potentiation (LTP) and spatial learning. In addition to its calcium-calmodulin (CaM)-dependent activity, this protein can undergo autophosphorylation, resulting in CaM-independent activity. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2008]
Catalog No
A7033
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF/ICC, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CAMK2A; CAMKA; KIAA0968; Calcium/calmodulin-dependent protein kinase type II subunit alpha; CaM kinase II subunit alpha; CaMK-II subunit alpha; CAMK2D; CAMKD; Calcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II
Molecular Weight (Da)
54030
Gene Name
CAMK2A/CAMK2D
Protein Name
Calcium/calmodulin-dependent protein kinase type II subunit alpha/delta
Human Gene ID
815/817
Human Swiss Prot No.
Q9UQM7/Q13557
Immunogen
The antiserum was produced against synthesized peptide derived from human CaMK2 around the phosphorylation site of Thr286. AA range:256-305
Specificity
Phospho-CaMKIIα/δ (T286) Polyclonal Antibody detects endogenous levels of CaMKIIα/δ protein only when phosphorylated at T286.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Immunohistochemistry analysis of paraffin-embedded human brain, using CaMK2 (Phospho-Thr286) Antibody. The picture on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using CaMK2 (Phospho-Thr286) Antibody
