The scaffolding protein encoded by this gene is the main component of the caveolae Plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]
Catalog No
A7034
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
CAV1; CAV; Caveolin-1
Molecular Weight (Da)
20472
Gene Name
CAV1
Protein Name
Caveolin-1
Human Gene ID
857
Human Swiss Prot No.
Q03135
Immunogen
The antiserum was produced against synthesized peptide derived from human Caveolin-1 around the phosphorylation site of Tyr14. AA range:5-54
Specificity
Phospho-Caveolin-1 (Y14) Polyclonal Antibody detects endogenous levels of Caveolin-1 protein only when phosphorylated at Y14.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HUVEC cells treated with PMA 125ng/ml 30' and HeLa cells treated with LPS 100ng/ml 30', using Caveolin-1 (Phospho-Tyr14) Antibody. The lane on the right is blocked with the phospho peptide.
