cortactin(CTTN) Homo sapiens This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the Cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]
Catalog No
A7049
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Western blot analysis of lysates from HeLa cells treated with H2O2, using Cortactin (Phospho-Tyr421) Antibody. The lane on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Cortactin (Phospho-Tyr421) Antibody
Fan, Mengtian, et al. "CX3CL1 promotes tumour cell by inducing tyrosine phosphorylation of cortactin in lung cancer." Journal of Cellular and Molecular Medicine 25.1 (2021): 132-146.
Fan, Mengtian, et al. "CX3CL1 promotes tumour cell by inducing tyrosine phosphorylation of cortactin in lung cancer." Journal of Cellular and Molecular Medicine 25.1 (2021): 132-146.
Fan, Mengtian, et al. "CX3CL1 promotes tumour cell by inducing tyrosine phosphorylation of cortactin in lung cancer." Journal of Cellular and Molecular Medicine 25.1 (2021): 132-146.