The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Sep 2011]
Catalog No
A7051
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
PPP1R14A; CPI17; PPP1INL; Protein phosphatase 1 regulatory subunit 14A; 17 kDa PKC-potentiated inhibitory protein of PP1; Protein kinase C-potentiated inhibitor protein of 17 kDa; CPI-17
Molecular Weight (Da)
16693
Gene Name
PPP1R14A
Protein Name
Protein phosphatase 1 regulatory subunit 14A
Human Gene ID
94274
Human Swiss Prot No.
Q96A00
Immunogen
The antiserum was produced against synthesized peptide derived from human CPI17 alpha around the phosphorylation site of Thr38. AA range:5-54
Specificity
Phospho-CPI-17 (T38) Polyclonal Antibody detects endogenous levels of CPI-17 protein only when phosphorylated at T38.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from RAW264.7 cells, using CPI17 alpha (Phospho-Thr38) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CPI17 alpha (Phospho-Thr38) Antibody. The picture on the right is blocked with the phospho peptide.