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Primary Antibodies
CREB-1 (Phospho-Ser133) Antibody
CREB-1 (Phospho-Ser133) Antibody

Assay Biotechnology Inc Antibodies and ELISA Kits have been rigorously validated for laboratory grade quality results. High specificity paired with high affinity for targets of interest give researchers an advantage with our products

Catalog No: A7053
Reactivity:Human,Mouse,Rat,Pig
Applications:IF,WB,IHC,IP,ELISA
Modification:Phospho Specific
Source:Store at -20°C/1 year
Storage and Stability:

$205
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CREB-1 (Phospho-Ser133) Antibody

Immunofluorescence analysis of mouse-lung tissue. 1,CREB-1 (phospho Ser133) Polyclonal Antibody(red) was diluted at 1:200(4°C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

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Product Details
Product Name
CREB-1 (Phospho-Ser133) Antibody
Description
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms. [provided by RefSeq, Mar 2016],
Catalog No
A7053
Reactivity
Human, Mouse, Rat, Pig
Source
Polyclonal, Rabbit,IgG
Modification
Phospho Specific
Applications
IF, WB, IHC, IP, ELISA
Dilution
IF 1:50-200 WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 ug:mg lysate. ELISA: 1:10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
Store at -20°C/1 year
Other Name
CREB1; Cyclic AMP-responsive element-binding protein 1; CREB-1; cAMP-responsive element-binding protein 1
Molecular Weight (Da or kDa)
Gene Name
CREB1
Protein Name
Cyclic AMP-responsive element-binding protein 1
Human Gene ID
1385
Human Swiss Prot No.
P16220
Immunogen
The antiserum was produced against synthesized peptide derived from human CREB around the phosphorylation site of Ser133. AA range:100-149
Specificity
Phospho-CREB-1 (S133) Polyclonal Antibody detects endogenous levels of CREB-1 protein only when phosphorylated at S133.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
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Publications (10)
Tuftelin 1 Facilitates Hepatocellular Carcinoma Progression through Regulation of Lipogenesis and Focal Adhesion Maturation WB,IF Human 1 : 1000, 1:100 /Huh7 cells, MHCC97H cells

Author: Available in link provided

Date of Publication : Available in link provided

Calcium/Calmodulin-Dependent Protein Kinase IV Mediates IFN-γ-Induced Immune Behaviors in Skeletal Muscle Cells. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY 2018 Mar 22 WB Mouse 1:1000 brain myogenic precursor cells (MPCs), C2C12 cell

Author: Available in link provided

Date of Publication : Available in link provided

Pituitary miRNAs target GHRHR splice variants to regulate GH synthesis by mediating different intracellular signalling pathways. RNA Biology Rna Biol. 2020;17(12):1754-1766 WB Pig,Rat primary pituitary cell,GH3 cell

Author: Available in link provided

Date of Publication : Available in link provided

Cyclooxygenase-2 up-regulates hepatic somatostatin receptor 2 expression. Scientific Reports Sci Rep-Uk. 2018 Jul;8(1):1-11 WB Rat,Human cirrhotic liver L02 cell

Author: Available in link provided

Date of Publication : Available in link provided

Pirfenidone decreases mesothelioma cell proliferation and migration via inhibition of ERK and AKT and regulates mesothelioma tumor microenvironment in vivo. Scientific Reports 2018 Jul 03 WB Human JL-1 cell,H2052 cell

Author: Available in link provided

Date of Publication : Available in link provided

Fetal exposure to dichloroacetic acid and impaired cognitive function in the adulthood. Brain and Behavior Brain Behav. 2020 Oct;10(10):e01801 WB Rat 1 : 1000 Hippocampal tissue

Author: Available in link provided

Date of Publication : Available in link provided

Dichloroacetic acid-induced dysfunction in rat hippocampus and the protective effect of curcumin. METABOLIC BRAIN DISEASE Metab Brain Dis. 2021 Apr;36(4):545-556 WB Rat 1:800 Hippocampus

Author: Available in link provided

Date of Publication : Available in link provided

β2-microglobulin has a different regulatory molecular mechanism between ER + and ER − breast cancer with HER2 −. BMC CANCER Bmc Cancer. 2019 Dec;19(1):1-11 IHC Human 1:150 breast cancer tissue

Author: Available in link provided

Date of Publication : Available in link provided

miR-709 inhibits GHRP6 induced GH synthesis by targeting PRKCA in pituitary. MOLECULAR AND CELLULAR ENDOCRINOLOGY Mol Cell Endocrinol. 2020 Apr;506:110763 WB Rat GH3 cell

Author: Available in link provided

Date of Publication : Available in link provided

Tanshinone IIA Improves Depression-like Behavior in Mice by Activating the ERK-CREB-BDNF Signaling Pathway. NEUROSCIENCE Neuroscience. 2020 Mar;430:1 WB Mouse brain

Author: Available in link provided

Date of Publication : Available in link provided

Primary Antibodies

    • ELISA Kits

    • Research Areas

    Cell Adhesion Proteins Cell Cycle Proteins Channel Proteins Exosome GDP/GTP Binding Proteins Growth Factors and Hormones Homeodomain Proteins Immunohistochemical Kinases and Phosphatases Signaling

     

    Membrane Receptors Neurobiology Signaling Intermediates Steroid Receptors Structural Proteins Synthesis and Degradation Transcription Regulators Transport and Trafficking Tumor Suppressors/Apoptosis
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