docking protein 1(DOK1) Homo sapiens The protein encoded by this gene is part of a signal transduction pathway downstream of receptor tyrosine kinases. The encoded protein is a scaffold protein that helps form a platform for the assembly of multiprotein signaling complexes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]
Catalog No
A7058
Reactivity
Human, Mouse, Rat
Applications
WB, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
DOK1; Docking protein 1; Downstream of tyrosine kinase 1; p62(dok); pp62
Molecular Weight (Da)
52392
Gene Name
DOK1
Protein Name
Docking protein 1
Human Gene ID
1796
Human Swiss Prot No.
Q99704
Immunogen
The antiserum was produced against synthesized peptide derived from human p62 Dok around the phosphorylation site of Tyr398. AA range:365-414
Specificity
Phospho-Dok-1 (Y398) Polyclonal Antibody detects endogenous levels of Dok-1 protein only when phosphorylated at Y398.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from K562 cells treated with Starvation 24h, using p62 Dok (Phospho-Tyr398) Antibody. The lane on the right is blocked with the phospho peptide.
Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using p62 Dok (Phospho-Tyr398) Antibody