The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
Catalog No
A7066
Reactivity
Human, Mouse, Rat
Applications
IF/ICC, WB, IHC-p, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Western blot analysis of lysates from K562 cells treated with IFN-alpha 1000U/ml 18h, using eIF2 alpha (Phospho-Ser51) Antibody. The lane on the right is blocked with the phospho peptide.
Western blot analysis of lysates from 1) KB, 2) MCF-7 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody(ABT:SA0439)was diluted at 1:10000, 37° 1hour. (Red) Tubulin β Monoclonal Antibody(5G3) (ABT:N1294) antibody was diluted at 1:5000 as loading control, 4° over night,Dylight 680 secondary antibody(ABT:SA0436)was diluted at 1:10000, 37° 1hour.
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Lei, Ting, et al. "Stress kinases, endoplasmic reticulum stress, and Alzheimer’s disease related markers in peripheral blood mononuclear cells from subjects with increased body weight." Scientific reports 6 (2016): 30890.
