This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14. [provided by RefSeq, Mar 2012]
Catalog No
A7068
Reactivity
Human, Mouse, Rat
Applications
WB, IHC-p, IF(paraffin section), IP, ELISA
Modification
Phospho Specific
Source
Polyclonal Rabbit
Dilution
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration
1 mg/ml
Storage and Stability
-20°C/1 year
Other Name
ELK1; ETS domain-containing protein Elk-1
Molecular Weight (Da)
44888
Gene Name
ELK1
Protein Name
ETS domain-containing protein Elk-1
Human Gene ID
2002
Human Swiss Prot No.
P19419
Immunogen
The antiserum was produced against synthesized peptide derived from human Elk1 around the phosphorylation site of Ser383. AA range:351-400
Specificity
Phospho-Elk-1 (S383) Polyclonal Antibody detects endogenous levels of Elk-1 protein only when phosphorylated at S383.
Formulation
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Western blot analysis of lysates from HeLa cells treated with UV, using Elk1 (Phospho-Ser383) Antibody. The lane on the left is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Elk1 (Phospho-Ser383) Antibody. The picture on the right is blocked with the phospho peptide.
